Pharmaceutical allergy pattern for antibacterial of some specified species of bacterial which diagnosed by vitek-2 technology isolated from the infection ... قراءة المزيد

ABSTRACT : The paper describes a field survey performed to determine the prevalence of hydatid cyst disease in cattle at slaughters in Al-Diwanyah abattoir. The study lasted for five months from November 2017 to April 2018. During the study, 200 cattle were examined. The study included 15 samples were collected from fluids and the germinal layer. The isolation and identification of E. granulosus were done using microscopic visualization followed by confirmation using a polymerase chain reaction (PCR) technique targeting the antigen B (AgB2) gene. The results indicate that(7.5%) of cattle were infected. The livers of cattle demonstrated a higher incidence of hydatid cysts than the lungs, with 6.66% oflivers and 20% of lung being infected, respectively. Also, the results of the study showed that the sex of the slaughtered animals has non-significant effect on the distribution of HC, as the rate of HC in males and females were close. The microscopic results revealed the presence of characteristic cysts. All E. granulosus isolates in cows appeared to be contained this gene show one distinct band (MW400 bp) when electrophoresed on agarose gel. The results of this study indicated that the PCR technique had a high specify in the detection of E. granulosus especially this species that encoded to AgB2 gene isolated from cowsin comparison to other routine diagnostic tests. ... قراءة المزيد