هبة محمد شهاب

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Almost all livestock animals are susceptible to ticks considered vectors and reservoirs for many tick-borne pthogenes, causing severe and fatal zoonotic diseases affecting humans and animals' health, such as Crimean Congo hemorrhagic fever (CCHF) virus. Due to this importance, ticks must be characterized using different tools, such as molecular, which helps epidemiological and control programs related to ticks. The current study was conducted during September 2018 and January 2019; fifty ticks were collected from sheep raised in Al-Saniyah territory, Al-Diwaniyah Province, Iraq. Then, the ticks were classified according to morphology, and 20 ticks were identified using polymerase chain reaction (PCR) to confirm the morphological specifications using the cytochrome c oxidase subunit I (COX1) gene as a target. Finally, six purified PCR products were conducted to a partial gene sequencing (PGS) method to identify the tick species and compare them to the global isolates. The morphological identification showed that ticks appeared to be from the genus Hyalomma, which was PCR-confirmed. Furthermore, the PGS revealed that the local tick isolates belonged to Hyalomma anatolicum, and they were firmly nucleotide-similar to strains from Pakistan and China. The results of the phylogenetic analysis showed probable evolutionary links to the Pakistanian and Chinese isolates. ... قراءة المزيد

هبة محمد شهاب

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Aim of our study is investigation of the genetic relationships between Salmonella spp isolates which taken from human stool isolates and Salmonella spp isolates which taken from cattle meat. The total number of study isolates is one hundred (50 cattlebeef and 50human stools. Bacterial isolation and bacterial identification are used for testing the samples for culturing and examined by biochemical tests then detected by using 16S rRNA gene in PCR that designed by NCBI and make DNA sequencing for two positive isolates. Our results revealed that percentage of Salmonellaspp was 8/50(16%) which isolated from cattle beef and was 10/50(20%) which isolated from human stool. The PCR was demonstrated highly specific and sensitive. DNA sequencing of part of 16S rRNA was show highly similarity with NCBI-Blast data and the phylogenetic study was reveal genetic homology at (0.5 genetic change) between animals and human isolates and then the gene sequence deposited into NCBI-Genbank accession numbers accession numbers (MN523665.1) and (MG388337.1). In conclusion, our study is done for first-time in Iraq of the genetic correlating between Salmonellaspp which isolated from cattle meat and human stool. So, it is important to know the cattle meat is significant source for spreading infection between human and animals. ... قراءة المزيد

البحوث المستلة لطالبة الماجستير فاطمة فليح عودة/كلية الادراة والاقتصاد/قسم المحاسبة

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1-اثر اختبار مكونات التنوع الثقافي في السلوك المحاسبي لتحسين الأداء المالي :دراسة مقارنة                                                   في جمهورية العراق والاردن 2-The Impact ... قراءة المزيد