{"id":32787,"date":"2021-08-06T19:18:07","date_gmt":"2021-08-06T19:18:07","guid":{"rendered":"https:\/\/qu.edu.iq\/repository\/?p=32787"},"modified":"2021-08-06T19:18:07","modified_gmt":"2021-08-06T19:18:07","slug":"%d9%87%d8%a8%d8%a9-%d9%85%d8%ad%d9%85%d8%af-%d8%b4%d9%87%d8%a7%d8%a8","status":"publish","type":"post","link":"https:\/\/repository.qu.edu.iq\/?p=32787","title":{"rendered":"\u0647\u0628\u0629 \u0645\u062d\u0645\u062f \u0634\u0647\u0627\u0628"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Molecular Sequencing Study of Salmonella spp Which Isolated from Cattle Meat and Human Stool<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Aim of our study is investigation of the genetic relationships between Salmonella spp isolates which taken<br \/>\nfrom human stool isolates and Salmonella spp isolates which taken from cattle meat. The total number<br \/>\nof study isolates is one hundred (50 cattlebeef and 50human stools. Bacterial isolation and bacterial<br \/>\nidentification are used for testing the samples for culturing and examined by biochemical tests then detected<br \/>\nby using 16S rRNA gene in PCR that designed by NCBI and make DNA sequencing for two positive<br \/>\nisolates. Our results revealed that percentage of Salmonellaspp was 8\/50(16%) which isolated from cattle<br \/>\nbeef and was 10\/50(20%) which isolated from human stool. The PCR was demonstrated highly specific<br \/>\nand sensitive. DNA sequencing of part of 16S rRNA was show highly similarity with NCBI-Blast data and<br \/>\nthe phylogenetic study was reveal genetic homology at (0.5 genetic change) between animals and human<br \/>\nisolates and then the gene sequence deposited into NCBI-Genbank accession numbers accession numbers<br \/>\n(MN523665.1) and (MG388337.1). In conclusion, our study is done for first-time in Iraq of the genetic<br \/>\ncorrelating between Salmonellaspp which isolated from cattle meat and human stool. So, it is important to<br \/>\nknow the cattle meat is significant source for spreading infection between human and animals. &#8230; <a class=\"cz_readmore\" href=\"https:\/\/repository.qu.edu.iq\/?p=32787\"><i class=\"fa fa-sign-out\" aria-hidden=\"true\"><\/i><span>\u0642\u0631\u0627\u0621\u0629 \u0627\u0644\u0645\u0632\u064a\u062f<\/span><\/a><\/p>\n","protected":false},"author":74,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[12],"tags":[],"class_list":["post-32787","post","type-post","status-publish","format-standard","hentry","category-12"],"_links":{"self":[{"href":"https:\/\/repository.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/32787","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/repository.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/repository.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/repository.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/users\/74"}],"replies":[{"embeddable":true,"href":"https:\/\/repository.qu.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32787"}],"version-history":[{"count":0,"href":"https:\/\/repository.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/32787\/revisions"}],"wp:attachment":[{"href":"https:\/\/repository.qu.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32787"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/repository.qu.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32787"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/repository.qu.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32787"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}